Journal: Journal of the American Society of Nephrology : JASN

Article Title: Genomic Analysis of Kidney Allograft Injury Identifies Hematopoietic Cell Kinase as a Key Driver of Renal Fibrosis

doi: 10.1681/ASN.2016020238

Figure Lengend Snippet: Inhibiting Hck in vivo attenuates kidney fibrosis by TGF-β1/SMAD3 signaling in a murine UUO model. UUO was performed on male C57BL/6 mice, gavaged with dasatinib or vehicle. Mice were perfused 3 or 7 days later. (A) Scheme of the experimental approach is shown. (B) WBs of phospho-SMAD3, t-SMAD3 from mouse kidney cortex lysates at 7 days post-UUO are shown for kidneys of PBS and dasatinib-gavaged mice. (C) Immunohistochemistry for anti-Hck (phosphorylation Y410) antibody in mouse kidney confirming dasatinib inhibiting HCK’s activation. Original magnification, ×200. Also, (D) Hck transcription level was increased in 3 and 7 days UUO kidneys by RT-PCR (normalized to GAPDH). (D) mRNA-levels of profibrotic markers at 7 days post-UUO by RT-PCR (normalized to GAPDH), from whole cortices of control and UUO kidneys of dasatinib- and PBS-fed animals (n=5). (E) Representative ×40 images from control and UUO kidneys of dasatinib- and PBS-gavaged mice at 7 days post-UUO. Picrosirius red stain (top row), and COL1A1 IF (middle row, red: COL1A1; blue: DAPI), and a-SMA IF (lower row, red: a-SMA; blue: DAPI) are depicted here. (F, G, and H) Morphometric quantification of thresholded ×40 images (n=6 animals; ten random hpfs per animal), showing area of (F) picrosirius red staining, (G) COL1A1 IF, and (H) a-SMA IF as a percentage of total hpf area. (B, C, D, F, G, and H) Values are mean±SEM; *P=0.05, **P<0.001, ***P<0.001 between means; one-way ANOVA with Bonferroni multiple comparison test.

Article Snippet: For IF, snap-frozen kidney sections were formalin-fixed and treated with anti-COL1A1 antibody (1:100 dilution; catalog no. 1310–01; Southern Biotech) overnight, and subjected to fluorescence microscopy.

Techniques: In Vivo, Immunohistochemistry, Phospho-proteomics, Activation Assay, Reverse Transcription Polymerase Chain Reaction, Control, Staining, Comparison

Journal: eLife

Article Title: A survey of optimal strategy for signature-based drug repositioning and an application to liver cancer

doi: 10.7554/eLife.71880

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Collagen I (Rabbit polyclonal) , ProteinTech , Cat#: 14695-1-AP; RRID: AB_2082037 , WB (1:2000)IF (1:200).

Techniques: Sequencing, Software

Journal: Diagnostics

Article Title: Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

doi: 10.3390/diagnostics10040246

Figure Lengend Snippet: Comparison of mRNA expression in paranasal sinus mucosa from the controls, and CRSsNP and CRSwNP patients as detected by RT-PCR. ( a ) scavenger receptor class B type 1 (SR-B1) and ( b ) lectin-like oxidized LDL receptor-1 (LOX-1) mRNA levels were quantitatively normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA levels. Center lines: median values. Boxes: interquartile ranges. Error bars: overall ranges. NS: not significant.

Article Snippet: The primary antibodies used were antihuman SR-B1 rabbit polyclonal antibody (#5193; ProSci, Poway, CA, USA), antihuman LOX-1 rabbit polyclonal antibody (#11837-1-AP; Proteintech, Rosemont, IL, USA), and antihuman CD68 mouse monoclonal antibody (#M0814; Dako, Glostrup, Denmark).

Techniques: Comparison, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Diagnostics

Article Title: Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

doi: 10.3390/diagnostics10040246

Figure Lengend Snippet: Correlation between the severity of computed tomography (CT) findings and mRNA expression levels for ( a ) SR-B1 and ( b ) LOX-1 in sinus mucosa.

Article Snippet: The primary antibodies used were antihuman SR-B1 rabbit polyclonal antibody (#5193; ProSci, Poway, CA, USA), antihuman LOX-1 rabbit polyclonal antibody (#11837-1-AP; Proteintech, Rosemont, IL, USA), and antihuman CD68 mouse monoclonal antibody (#M0814; Dako, Glostrup, Denmark).

Techniques: Computed Tomography, Expressing

Journal: Diagnostics

Article Title: Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

doi: 10.3390/diagnostics10040246

Figure Lengend Snippet: Representative immunohistological images showing SR-B1 ( a , b ), LOX-1 ( c , d ), and CD68 ( e , f ) expression in ethmoid sinus mucosa sampled from a CRSwNP patient. Vascular endothelial cells (arrowheads) are stained positively both for SR-B1 and LOX-1. In contrast, numerous submucosal inflammatory cells show intense positive staining for LOX-1 compared to that for SR-B1. Scale bar: 20 μm.

Article Snippet: The primary antibodies used were antihuman SR-B1 rabbit polyclonal antibody (#5193; ProSci, Poway, CA, USA), antihuman LOX-1 rabbit polyclonal antibody (#11837-1-AP; Proteintech, Rosemont, IL, USA), and antihuman CD68 mouse monoclonal antibody (#M0814; Dako, Glostrup, Denmark).

Techniques: Expressing, Staining